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janelia7_blocks-janelia7_biblio_header | block
Nature Methods. 2020 Jan 01;17(1):68-71. doi: 10.1038/s41592-019-0641-2
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution. Hess Lab
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Hayworth KJ, Peale D, Januszewski M, Knott GW, Lu Z, Xu CS, Hess HF
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Abstract
We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.
PMID: 31740820 [PubMed - indexed for MEDLINE]
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Janelia Authors
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